Mickey & Minnie's Runaway Railway

For the installation at Disney's Hollywood Studios, guests enter through a full-sized re-creation of Grauman's Chinese Theatre.[22] The line queue winds through the lobby before entering the auditorium, and the walls are adorned with a variety of film posters that depict fictitious Mickey Mouse short films that premiered at the theater.[23] In addition, the posters artwork depicting actual Mickey Mouse shorts: "Croissant de Triomphe", "Mumbai Madness", "Potatoland", "Split Decision", "Three-Legged Race", "Tokyo Go", "Wish Upon a Coin", "Wonders of the Deep", and "Yodelberg". Each poster was created specifically for this attraction by the Disney Television Animation.

For the upcoming installation at Disneyland, the ride will be housed in a new building within Mickey's Toontown called the El CapiTOON Theater, a pun of the El Capitan Theatre in Hollywood which in turn is owned by Disney. The queue for this version will feature an exhibit designed by the fictitious Toontown Hysterical Society, showcasing elements of the "toon world" including costumes and other props.[24]

After entering the auditorium, guests are introduced to a new short film premiere from Mickey and Minnie called Perfect Picnic. The animated cartoon short begins with Mickey, Minnie, and Pluto preparing for a picnic at Runnamuck Park, singing "Nothing Can Stop Us Now". While packing Mickey's car, Minnie accidentally packs Pluto with the picnic basket in the trunk. The duo make their way to the park. Along the way, Mickey and Minnie drive alongside Goofy, who is operating the Runnamuck Railroad train that travels around the park. Their car hits a bump as it moves over a railroad crossing, popping open the trunk and launching Pluto along with the picnic basket into the air. Pluto manages to save the basket, but a pie falls out and lands on the train's smokestack. With the smokestack clogged, the train picks up speed. Goofy panics as the train pulls into a railroad barn, where an explosion occurs, causing a large, ragged hole to appear in the movie screen, thus literally breaking the fourth wall. When the smoke clears, Goofy lands on a loose board on the outside of the barn, sees the guests and invites them to ride with him on the train. He asks a nearby cast member to help the guests step inside the cartoon while he returns to the barn to fix the train just after he tells them he'll be back to pick them up. After walking through the movie screen, guests find themselves in the cartoon railroad station and make their way to the loading platform where the train arrives.

Once the train stops at the loading station, guests board one of four cars behind the locomotive. The train then departs the station and makes a left turn, passing the park on a musical sunny day before entering a tunnel. Once inside, Goofy opens the locomotive's back window, asking guests if they are ready for a tour around the park. Mickey and Minnie pull alongside the train and exchange greetings. Mickey parks next to a railroad switch lever and asks Goofy to take care of the guests. Goofy proudly exclaims, "Gawrsh, they're with me, what could possibly go wrong? Well, back to work." Goofy closes his window afterwards and begins to sing "I've Been Working on the Railroad" when the nearby switch lever falls, disconnecting the cars from Goofy's locomotive while Mickey realizes he drove into it. The locomotive makes a left, while the cars with guests careen to the right. Mickey and Minnie race after the runaway train.

The cars exit the tunnel into a desert with Mickey and Minnie on horses trying to round up the cars. Despite their attempts to help, Mickey and Minnie become tangled in their ropes, with nearby vultures roosting nearby. The cars then enter an amusement park where Mickey and Minnie float in attached to some balloons. Before they can make their way down, the twister logo on the side of the park's wooden roller coaster comes to life, sending everything into a frenzy. This tornado is an homage to the Fantasia scene formerly occupying the same space, (this itself was originally planned to be the tornado from The Wizard of Oz). Each car moves into a dark room with the tornado at its center. Mickey, Minnie, and Pluto are seen swirling around. In the next room, the duo lands on a tropical island where a volcanic eruption occurs. Each of the four cars enter a scene where Mickey and Minnie are shown to be caught in a rapid downstream heading toward a waterfall. Guests are seemingly following close behind and fall with them into the water, emerging moments later in an underwater reef. It is filled with a variety of animated sea life moving to doo-wop music with some playing instruments and including a blue squid playing his trumpet.

The scene transitions into a sewage system where the water drains away, and the cars move into another room depicting the downtown of a large city at night. Pete can be seen with a jackhammer doing construction work and laughing, while Donald Duck is spotted in a delivery van honking at the traffic the train cars are causing. The cars veer left into a room designed as a dance studio, and Daisy Duck leads them into a waltz followed by a conga. The cars dance their way into a back alley and into a factory, as Minnie warns not to enter. The cars then appear to be stuck on a conveyor belt moving toward a smashing machine. Mickey and Minnie eventually manage to pull a switch that shuts down the factory and transforms it into a moonlit night at the park. The cars reverse and turn around, moving into another room where Mickey, Minnie, and Pluto are finally having their picnic. The lead car reattaches to the locomotive helmed by Goofy, who tells guests that his "guided tour" has concluded and thanks them for staying with him. Goofy waves goodbye, closes the back window and is heard discovering a lever wondering what it does. Just before the train returns to the station, he pulls it, triggering a small explosion inside his cab followed by his signature holler, giving riders one last laugh. Guests leave the station and pass through the same movie screen returning them to the real world, which now shows "The End" title card.

During the COVID-19 pandemic, plexiglass was installed between rows to protect guests from exposure to COVID-19 and increase capacity. The pre-show was suspended and a voice-over plays through the queue while guests pass through the pre-show room. The full pre-show experience was restored on June 14, 2021, as part of the resumption of normal operations throughout Walt Disney World.[25]